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Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Quantity

配制成每升培養基含 4.4 克粉末。

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Murashige and Skoog Basal Medium
Catalog Number M5519
Storage Temperature 2–8 °C
Product Description
Classic plant cell culture medium containing macro and
micronutrients, and vitamins.1
Components mg/L
Ammonium nitrate 1,650.0
Boric acid 6.20
Calcium chloride (anhydrous) 332.20
Cobalt chloride hexahydrate 0.0250
Cupric sulfate pentahydrate 0.0250
Disodium EDTA dihydrate 37.260
Ferrous sulfate heptahydrate 27.80
Glycine 2.0
Magnesium sulfate (anhydrous) 180.70
Manganese sulfate monohydrate 16.90
myo-Inositol 100.0
Nicotinic acid 0.50
Potassium iodide 0.830
Potassium nitrate 1,900.0
Potassium phosphate monobasic 170.0
Pyridoxine hydrochloride 0.50
Sodium molybdate dihydrate 0.250
Thiamine hydrochloride 0.10
Zinc sulfate heptahydrate 8.60
4.4 g of powder are required to prepare 1 L of medium.
Precautions and Disclaimer
This product is for R&D use only, not for drug,
household, or other uses. Please consult the Material
Safety Data Sheet for information regarding hazards
and safe handling practices.
Preparation Instructions
Do not open the container until the contents are allowed
to warm to room temperature. If possible the entire
contents of the package should be used immediately
after opening. Preparing this product in a concentrated
form is not recommended as some salt complexes may
precipitate. Supplements may be added prior to
sterilization or added aseptically to the sterile medium.
Certain supplements (i.e., heat labile) may require filter
sterilization and may affect the shelf life of the medium.
The basic steps for preparing culture medium are the
following:
1. Using a container twice the size of the desired final
volume, measure out ~90% of the required final
volume of tissue culture grade water (e.g., Catalog
Number W3500). Example: 900 ml for a final
volume of 1000 ml.
2. While stirring, add the powdered product.
3. Rinse the original container with a small volume of
tissue culture grade water to remove traces of the
powder. Add to the solution in step 2.
4. Add desired supplements (e.g., sucrose, gelling
agent, auxins, cytokinins).
5. While stirring, adjust to the desired pH (e.g.,
5.7±0.1) using KOH, NaOH, or HCl.
6. Add additional tissue culture grade water to bring
the medium to the final volume.
7. If a gelling agent is used heat the solution to clarity
while stirring.
8. Dispense the medium into culture vessels before or
after autoclaving according to the application. Add
heat labile constituents after autoclaving.
9. Sterilize the medium in a validated autoclave at
1 kg/cm2 (15 psi). The medium should attain a
temperature of 121 °C for at least 15 minutes.
Storage/Stability
All media preparations should be stored at 2–8 °C.
Store dry powder in a desiccator as the powder is
extremely hygroscopic and must be protected from
atmospheric moisture. Deterioration of powdered
medium may be recognized by:
1. color change
2. granulation, clumping, or particulate matter
throughout the powder
3. insolubility
4. pH change
5. inability to promote growth when properly used.
References
1. Murashige, T., and Skoog, F., Physiol. Plant, 15,
473-497 (1962).
MAM 02/10-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser
must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of
the invoice or packing slip.

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