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價格：電議
所在地：上海
型號：查詢甄準
更新時間：2020-09-27
瀏覽次數：809
公司地址：上海市閔行區浦江鎮三魯公路3398號國茸1號樓B-506室
陳玲(女士) 銷售工程師
上海甄準生物科技有限公司（簡稱甄準®）專業提供科研試劑產品，主要包括高端化學試劑、生物學試劑、分析標準品、對照品、標準物質等，應用覆蓋藥物分析領域、食品安全領域、聚合物研究領域、環境監測領域，客戶廣泛分布于食品、制藥、政府機構、第三方檢測、環境測試機構、化工、科研院校、生物工程等行業。
上海甄準生物擁有充滿活力的團隊，持續創新，致力于為客戶提供高質量產品和優質的服務。
*企業文化： 用心甄選，追求jing準。
*服務理念：真心傾聽您每一個標準。
*產品定位：甄準®科研試劑供應商。
*品牌形象：甄準®（代理、經銷國外進口原裝產品線）、zzstandard®（中藥植物提取物產品線）、GlycoSci®（糖/碳水化合物產品線）、IsoReag®（穩定性同位素標記物產品線）。
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Striving for high quality products and immediate service, we, SHANGHAI ZZBIO CO., LTD, shall do our best to ensure that your experiments and projects will be successful.
Our core business is to supply quality reference standard materials, high purity bio-chem reagents and technical consultancy to laboratories, universities, research institutions, hospitals, pharmaceutical companies, and drug manufacturers. These products can be supplied in quantities from a few milligrams to grams, and even to kilograms.
If you have special requirements, please contact us freely. We have considerable experience in custom-made products and will be very glad to discuss your needs.
甄準生物將一如既往，關注您的研發生產項目，為您提供zui高性價比產品和服務。
產品貨號：ZZK-K-ACETAF
產品品名：乙酸[AF法]檢測試劑盒
英文品名：ACETIC ACID(Acetyl-CoA Synthetase Format)assay Kit
規格型號：
(141.6 mL of reagent [R1 + R2] per kit;
equivalent to 456 reactions of 0.31 mL)
INTRODUCTION:
The most widely used method for enzymatic quantification of acetic acidis that based on the use of acetyl-coenzyme A synthetase (ACS),according to equations 1 – 3 below. For auto-analyser applications itbecomes necessary to prepare a “master mix” reagent (R1), containing allcomponents of the assay except the reaction initiation enzyme, ACS.However, reagent R1 when prepared from some kits has very limitedon-machine stability, owing to a rapidly increasing absorbance value. Toovercome this issue, Megazyme developed this kit (K-ACETAF), thatdoesn’t exhibit this instability phenomenon. Additionally, theanti-inhibitory compound polyvinylpyrollidone (PVP) has also beenincorporated into the assay to prevent inhibition caused by certaintannins found in grape juice and wine.
KITS:
Kits suitable for the preparation of 141.6 mL of reagent (equivalent to456 reactions of 0.31 mL) are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: Buffer (30 mL, pH 8.4) plus L-malic acid, PVP and sodiumazide (0.02 % w/v) as a preservative. Stable for > 2 years at 4°C.
Bottle 2: (x2) NAD+ plus ATP and CoA.Freeze dried powder.Stable for> 5 years at -20°C.
Bottle 3: L-Malate dehydrogenase plus citrate synthase suspension, 2.2mL. Stable for > 2 years at 4°C.
Bottle 4: Acetyl-coenzyme A synthetase suspension (1.1 mL). Stable for> 2 years at 4°C.
REAGENT PREPARATION:
Preparation of R1:
Component Volume
bottle 1 (buffer) 5.50 mL
bottle 2 (NAD+/ATP/CoA) 2.20 mL (after adding 5.50 mL of H2O to bottle 2)
bottle 3 (L-MDH/CS) 0.44 mL (swirl to mix before use)
H2O 18.35 mL
Total volume 26.49 mL
Preparation of R2:
Component Volume
bottle 4 (ACS) 0.22 mL (swirl to mix before use)
H2O 1.60 mL
Total volume 1.82 mL
EXAMPLE METHOD:
R1: 0.290 mL
Sample: ~ 0.005 mL
R2: 0.020 mL
Reaction time: 15 min at either 20-25°C or 37°C
Wavelength: 340 nm
Prepared reagent stability: > 3 days when refrigerated
Calculation: endpoint
Reaction direction: increase
Linearity: up to 30 μg/mL of acetic acid in final reaction solution
溫馨提示：需要詳細的產品使用說明書，請聯系甄準生物：
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上海甄準生物科技有限公司
熱線：
詹小龍 上海甄準生物科技有限公司
：
郵箱：3001080321@qq.com
直線 -204
地址：上海市浦東新區滬南路2419弄30號710室
郵編：201204
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免責聲明：以上所展示的[查詢甄準 乙酸[AF法]檢測試劑盒]信息由會員[上海甄準生物科技有限公司]自行提供，內容的真實性、準確性和合法性由發布會員負責。
