產品貨號：ZZK-K-AGLUA

產品品名：α-葡萄糖醛酸酶檢測試劑盒

英文品名：α-D-Glucuronidase assay Kit

規格型號：50 Assays per kit

A simple, reliable and accurate method for the measurement ofalpha-D-glucuronidase in various enzyme preparations. Containing andaldouronic acid substrate and an α-D-glucuronidase control.

INTRODUCTION:

After cellulose, xylan is the second most abundant polysaccharide

found in nature. Xylan is a hemicellulose comprising a β-(1,4)-

linked xylan back bone substituted with various side chains such as

acetyl groups, α-(1-2/3)-linked L-arabinofuranose and α-(1-2)-linked

4-O-methylglucuronic acid. A repertoire of enzymes are required to

effect the complete degradation of plant cell wall polysaccharides in

the utilisation of plant biomass. Hence, complete degradation of xylan

requires the concerted action of various enzymes including endo-β-

(1,4)-xylanases, β-(1,4)-xylosidases, acetylxylan esterases,

α-L-arabinofuranosidases and α-glucuronidases.

The importance of carbohydrate degrading enzymes has increased

over recent years due to the growing focus of obtaining renewable

energy from plant biomass in the form of biofuels such as cellulosic

and hemicellulosic ethanol. Various research programmes are striving

to develop optimised enzyme mixtures or modified microorganisms

to increase the efficiency of biomass conversion and, as such, it is

important that the various enzyme activities can be easily measured.

Simple methods of measuring enzyme activity for some of the well

studied carbohydrate degrading enzymes such as endo-β-(1,4)-

cellulases, endo-β-(1,4)-xylanases, β-(1,4)-xylosidases exist. However

for some enzymes such as α-glucuronidases, current available methods

are cumbersome.

This kit (K-AGLUA) provides a simple method suitable for the specific

measurement of α-D-glucuronidase activity (α-D-glucosiduronate

glucuronohydrolase; EC 3.2.1.139 and xylan α-1,2-glucuronosidase;

EC 3.2.1.131).

KITS:

Kits suitable for performing 50 assays in manual format (or 200 assays

in microplate format) are available from Megazyme. The kits contain

the full assay method plus:

Bottle 1: Aldouronic acid mixture (tri:tetra:penta) (6 mL;

10 mg/mL).

Stable for > 5 years at -20°C.

Bottle 2: α-D-Glucuronidase solution (2 mL; ~ 1.0 U/mL, see

label on bottle).

Stable for > 2 years at -20°C.

Bottle 3: Stop Buffer (28 mL, pH 10) plus sodium azide

(0.02 % w/v) as a preservative.

Stable for > 2 years at 4°C.

Bottle 4: NAD+ freeze dried powder.

Stable for > 5 years at -20°C.

Bottle 5: Uronate dehydrogenase suspension (1.1 mL).

Stable for > 2 years at 4°C.

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