產品貨號：ZZK-K-EBHLG

產品品名：酵母葡聚糖酶檢測試劑盒

英文品名：Enzymatic Yeast Beta-Glucan Assay Kit

規格型號：50 assays per kit

An enzymatic procedure for the measurement of 1,3:1,6-beta-glucan inyeast. Also measures 1,3-Beta-glucan.

INTRODUCTION:

(1-3)-Beta-Glucans are widely distributed in nature, especially in algae,

fungi and yeast, but also in higher plants. They serve a variety of

biological functions. They form the major structural components of

cell walls, they act as storage carbohydrates and they sometimes play

a protective role by forming at specific sites in response to particular

stimuli such as wounding.1 Yeast Beta-glucan substantially enhances the

function of the immune system by activating macrophages, one of the

primary defences of the immune system. Literature indicates that the

potent anti-tumour properties of polysaccharide fractions extracted

from certain strains of mushrooms, in particular Grifola frondosa

also known as Maitake, can be attributed to linear 6-branched

(1-3)-Beta-glucan.2-4 Similar properties have recently been assigned to

(1-3)(1-4)-Beta-glucans from cereal grains (e.g. oats and barley).

The presence in wine of a particular high molecular weight

(~ 800 Kd) Beta-glucan, produced by the fungus, Botrytis cinerea (grey

rot) leads to clarification and filtration problems.5 Scleroglucan

(from Sclerotium rofsii), an industrial thickening agent with remarkable

thickening properties, has a very similar structure.

Megazyme offers an acid hydrolysis/enzymic procedure (K-YBGL)

for the determination of Beta-glucan in yeast and mushroom. That

method is based on acid hydrolysis to measure total Beta-glucan and

specific enzymic determination of α-glucan. Beta-Glucan is determined

by difference. A totally enzymic procedure specific for Beta-glucan has

been published by Danielson et al.6 The method employs Lyticase

from Sigma-Aldrich and exo-1,3-Beta-glucosidase/Beta-glucanase from

Megazyme. This method works well, but its widespread adoption

is limited by the high cost of Lyticase. In this booklet we describe

an alternate, simple enzymic procedure for the measurement of

(1-3)(1-6)-Beta-glucan in yeast. The method also gives quantitation

measurement of Beta-glucan in curdlan, laminarin and cereal Beta-glucan

preparations. It does not give quantitative measurement of Beta-glucan

in mushrooms, the reasons for which are currently being researched.

KITS:

Kits suitable for carrying out 50 assays are available from Megazyme.

The kits contain the full assay method plus:

Bottle 1: GlucazymeTM preparation (exo-1,3-Beta-glucanase,

endo-1,3-Beta-glucanase, Beta-glucosidase and chitinase

suspension), 2.2 mL.

Stable for 大于4 years at 4°C.

Bottle 2: GOPOD Reagent Buffer. Buffer (48 mL,

pH 7.4), p-hydroxybenzoic acid and sodium azide

(0.4% w/v).

Stable for 大于4 years at 4°C.

Bottle 3: GOPOD Reagent Enzymes. Glu cose oxidase

plus peroxidase and 4-aminoantipyrine. Freeze

dried powder.

Stable for 大于5 years at -20°C.

Bottle 4: D-Glu cose standard solution (5 mL, 1.5 mg/mL)

in 0.2% w/v benzoic acid.

Stable for 大于5 years at room temperature.

Bottle 5: Control yeast Beta-glucan preparation (~ 2 g,

Beta-glucan content stated on the bottle label).

Stable for 大于5 years at room temperature.

Bottle 6: Control starch preparation (~ 2 g, 96%

starch dwb).

Stable for 大于5 years at room temperature.

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