產品貨號：ZZK-K-XYLS

產品品名：木聚糖酶AX檢測試劑盒

英文品名：Xylanase (Xylazyme AX) Assay Kit

規格型號：200 assays per kit

For the measurement of endo-1,4-?-D-xylanase in enzyme preparations,bread improver mixtures and animal feeds. Contains Xylazyme AX Tabletsand xylanase enzyme controls (A. niger and Trichoderma longibrachiatum).

INTRODUCTION:

Arabinoxylan is the major endosperm cell-wall polysaccharide of

wheat and rye and is found in significant proportions in most cereal

solutions and slurries of high viscosity, and in animal nutrition it

reduces the rate of nutrient absorption from the gut.

endo-Beta-D-Xylanase (xylanase) is added to feeds to catalyse

depolymerisation of this polysaccharide. It can be demonstrated

that endo-cleavage by xylanase of just one bond per thousand in the

arabinoxylan backbone can significantly remove viscosity properties.

Of the carbohydrase enzymes used as feed supplements, one of the

most difficult to measure has been xylanase. These problems are

attributed to several factors, including the low levels of enzyme

added to the feed, inactivation of enzyme during pelleting, binding

of the enzyme to feed components and the presence of specific

xylanase inhibitors.

The only biochemical methods which are sufficiently sensitive, specific

and robust to measure xylanase in feeds are viscometric assays and

those employing dyed xylan or arabinoxylan polysaccharides.

Viscometric assays are tedious, whereas assays employing dyed

xylan substrates are rapid, reproducible and simple to perform.

We recommend the use of either Xylazyme AX tablets or Azo-

Wheat Arabinoxylan (Azo-WAX). Xylazyme AX based assays

are about 5-fold more sensitive than assays employing Azo-WAX.

However, this latter substrate does have sufficient sensitivity in most

applications, and results are slightly more reproducible than with

Xylazyme AX.

It is generally accepted that xylanase enzymes which are best suited

to feed applications have optimal activity at pH 6.0. Consequently,

these enzymes are generally assayed at this pH in 100 mM sodium

phosphate buffer. In recovery experiments, however, we found that

sodium phosphate buffer extracts only a small proportion (< 20%)

of the amount of enzyme added to the feed. Thus a wide range of

alternative extractants and extraction conditions have been evaluated.

For feeds containing Trichoderma sp. xylanases, the best and most

consistent results have been obtained using 100 mM acetic acid

or 100 mM sodium acetate buffer (pH 4.7) at room temperature.

Optimal extraction of Humicola sp. xylanases was achieved with a

buffer containing 100 mM MES buffer (pH 6.0) and 1 % w/v sodium

dodecyl sulphate (SDS).

KITS:

Kits containing the required reagents to measure xylanase in animal

feeds are available from Megazyme. These kits contain:

1. Xylazyme AX test tablets (200 tablets).

2. A. niger control xylanase (~ 295 mU/mL at 40°C and pH 4.7)

in 50 % (v/v) glycerol (activity stated on vial).

3. T. longibrachiatum control xylanase (~ 386 mU/mL at 40°C and

pH 6.0) in 50% (v/v) glycerol (activity stated on vial).

愛爾蘭Megazyme公司是知名的酶及酶法分析試劑盒的生產廠家，

其中大多數產品都被AOAC(Association of Official Analytical Chemists)、

AACC(American Association of Cereal Chemists)、ICC(InternationalAssociation for Cereal Science and Technology)、

EBC(European Brewing Convention)、RACI(Royal Australian Chemical Institute)等組織采用。

其中歐洲檢測果汁及葡萄酒等發酵產品基本都采用該公司產 品。

其中全線產品及相關檢測信息見：

該公司產品分以下系列：

1. 生物及食品酶法分析試劑盒 (Diagnostic Kits)

2. 試劑混合物(Reagent Mixtures)

3. 協同因子(Cofactors)

4. 糖酶片劑試驗(Carbohydrase Tablet Tests)

5. 蛋白酶片劑試驗(Protease Test Tablets)

6. 可溶性呈色底物(Soluble Chromogenic Substrates)

7. 不可溶性（交聯的）呈色底物(Insoluble (Crosslinked) Chromogenic Substrates)

8. 酶(Enzymes)

9. 植物凝血素(Lectins)

10. 多聚糖(Polysaccharides)

11. 低聚糖(Oligosaccharides)

12. 相關產品 (General)

其中產品被機構采用，建立檢測方法如下：

總淀粉檢測盒（Total Starch Assay Kit）

AOAC Method 996.11

AACC Method 76-13

ICC Standard Method no. 168

RACI Standard Method

總膳食纖維檢測盒（Total Dietary Fibre Assay Kit）

AOAC Method 985.29

AOAC Method 991.42

AOAC Method 993.19

AOAC Method 991.43

AACC Method 32.07

AACC Method 32.21

AACC Method 32.05

淀粉損失檢測盒（Starch Damage Assay Kit）

AACC Method 76-31

ICC Standard No. 164

RACI Standard Method

α-淀FEN酶檢測盒Ceralpha (α-Amylase) Assay Kit

AOAC Method 2002.01

ICC Standard No. 303

RACI Standard Method

CCFRA Flour Testing Working Group Method 0018

AACC - under evaluation.

Alpha淀FEN酶檢測盒Amylazyme (α-Amylase Assay)

AACC Method 22.05

RACI Standard Method

果聚糖檢測盒Fructan Assay Kit

AOAC Method 999.03

AACC Method 32-32

木聚糖酶AX片劑（Xylazyme AX Tablets）

Xylazyme AX Tablets have been widely adopted in the fermentation andfeeds industries for the measurement of Xylanase.

Beta-葡聚糖檢測盒Beta-Glucan (Mixed linkage) Assay Kit

AACC Method 32-23

EBC Methods 3.11.1, 4.16.1 and 8.11.1

AOAC Method 995.16

ICC Standard No. 166

RACI Standard Method

限糊精酶和支鏈淀FEN酶檢測片劑（Limit-Dextrizyme）

RACI Standard Method

Beta-葡聚糖酶檢測盒（Beta-Glucanase (Malt and Microbial) Assay Kit）

RACI Standard Method

Beta-淀FEN酶檢測盒（Betamyl (Beta-Amylase Assay Kit)）

RACI Standard Method.

木聚糖酶檢測底物（Azo-Wheat Arabinoxylan ）

Azo-Wheat Arabinoxylan, Azo-CM-Cellulose and Ceralpha methods for theassay of Xylanase, cellulase and ?-Amylase, respectively, have beenadopted by the UK silage industry.

抗性淀粉檢測盒（Resistant Starch Assay Kit）

AOAC Method 2002.02

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