產(chǎn)品貨號：ZZK-K-TREH

產(chǎn)品品名：海藻糖檢測試劑盒

英文品名：Trehalose Assay Kit

規(guī)格型號：100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser)

A simple method for the rapid and reliable measurement of trehalose infoods, beverages and other materials.

Suitable for manual, auto-analyser and microplate formats.

INTRODUCTION:

Trehalose is a naturally occurring disaccharide containing two Glu cose

molecules bound in an α,α-1,1 linkage. This structure results in a

chemically stable, non-reducing sugar with many important functional

characteristics. Trehalose is commonly found in nature, provides

a source of energy, and has been shown to be a primary factor in

stabilising organisms during times of freezing, drying and heating.

Trehalose is consumed as part of a normal diet in mushrooms, honey,

lobster, shrimp and foods produced using baker’s and brewer’s yeast.

Trehalose has been determined to be generally recognised as safe

(GRAS) as a multipurpose ingredient for all uses in food, including

as a sweetener, stabiliser, thickener and flavour enhancer, in general

accordance with current good manufacturing practices. It has a

uniquely high glass transition temperature for a disaccharide, is nonbrowning,

and will not hydrolyse at low pH or high temperatures.

This results in more stable colours and flavours in foods to which

it is added. Trehalose is 45% as sweet as sucrose when compared

in a 10% (w/v) solution and is stable at up to 94% relative humidity.

The low hygroscopic nature of trehalose dihydrate results in a freeflowing,

stable dry product. As a non-reducing sugar it does not

react with amino acids or proteins to initiate Maillard browning.

KITS:

Kits suitable for performing 100 assays in manual format (or 1100

assays in auto-analyser format or 1000 assays in microplate format)

are available from Megazyme. The kits contain the full assay method

plus:

Bottle 1: Buffer (25 mL, pH 7.0) plus magnesium chloride and

sodium azide (0.02% w/v) as a preservative.

Stable for 大于 2 years at 4°C.

Bottle 2: NADP+ plus ATP.

Stable for 大于 5 years at -20°C.

Bottle 3: Hexokinase plus Glu cose-6-phosphate

dehydrogenase suspension, 2.25 mL.

Stable for 大于 2 years at 4°C.

Bottle 4: Trehalase suspension (2.25 mL).

Stable for 大于 2 years at 4°C.

Bottle 5: D-Glu cose standard solution (5 mL, 0.4 mg/mL)

in 0.2% (w/v) benzoic acid.

Stable for 大于 4 years at room temperature.

Bottle 6: Trehalose dihydrate control (~ 2 g).

Stable for 大于 4 years at room temperature.

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